Part:BBa_K1357009

amilCP Reporter (RBS+Coding+Term)

This part consists of an RBS, the blue chromogenic protein, amilCP, and a double terminator. This part is intended to serve as a useful reporter to determine what level of expression the system in which it is incorporated into without the need for a fluorescent microscope.

Usage and Biology

In order to show that a protein encoded on a plasmid will be expressed under desired conditions, a chromogenic change can be observed using this part. Instead of going through the process of using a fluorescent protein that could be costly to visualize for teams with less resources available or in the case that the given fluorescent protein interferes with your desired protein, a team can simply clone this behind their desired to determine expression levels of their desired protein.

iGEM17_SJTU_BioX_Shanghai:

J23119+Target3+amilCP BBa_K2285015
J23119+Target1+amilCP BBa_K2285019

An improved part has been constructed. Since this part is a coding sequence, we added a RBS which is on the upstream of sfGFP BBa_K515005 and terminator BBa_B1006 by PCR. What's more, our device have a stem-loop structure(called Target) following the constitutive promoter J23119, to achieve further control of amilCP expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]