Device

Part:BBa_K173010

Designed by: Lorenzo Pasotti, Susanna Schiavi, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

Lactose/IPTG inducible system (PoPS output)

This BioBrick is an aTc input -> PoPS output device.

BBa_J23118 promoter drives the constitutive production of lacI repressor (BBa_C0012), which inhibits lac promoter (BBa_R0011) activity. When lactose or IPTG is added to the medium, it binds lacI and inhibits it. So, the PoPS output is a function of lactose/IPTG concentration.

Thanks to the hybrid lac promoter (BBa_R0011), designed taking the Plambda promoter (BBa_R0051) and substituting its cI (BBa_C0051) binding sites with two lacI binding sites, the behaviour of this device is not a function of glucose concentration because the wild type CAP binding sites are not present in this artificial lac promoter.

Pv A11 BBa K173010.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None