Part:BBa_K143012

Promoter veg: Constitutive Promoter for B. subtilis

Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. Pveg contains binding sites for the B. sutbilis major sigma factor, Sigma factor A 1. Pveg in B. subtilis utilises two binding sites to cause high expression of genes #2, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulation factor spoOA interacts with Pveg though it is not known how #3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Part Characterisation

Please see the testing constructs BBa_K143079 and BBa_K143082 for characterisation data.

References

<biblio>

1. 1 pmid=6181373
2. 2 pmid=6820069
3. 3 pmid=14651647

</biblio>