Generator

Part:BBa_K1073011

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)

Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)

This device is a combination of BBa_K1073001 and BBa_K1073000 and mediates an inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.

Usage and Biology

The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-butyryl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-butyryl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None