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Part:BBa_J70437

Designed by: Julie Norville   Group: Knight Lab   (2009-08-16)

Reverse oligo part for testing J70428, J70429, J70430

see complementary forward oligo part J70436

single stranded only

Melting temperature 45.8 using http://www.biophp.org/minitools/melting_temperature/demo.php


Simulates BBa_J70438 when used with BBa_J70437 to test BBa_J70428, BBa_J70429, and BBa_J70430

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 23
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 23
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 23
    Illegal SpeI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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