Part:BBa_I52002

ccdB and minimal pUC19 derived high copy origin

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

BBa_I52002 is the default part cloned into the BioBrick cloning site of the BioBrick base vector BBa_I51020 and by extension all BioBrick standard vectors constructed from BBa_I51020. BBa_I52002 is composed of two parts BBa_P1016 and BBa_I50022. BBa_P1016 is the positive selection marker ccdB used to facilitate assembly of BioBrick parts. BBa_I50022 is a minimal high copy pUC19-derived replication origin. BBa_I50022 enables the base vector to be propagated at high copy for easy plasmid DNA purification.

Several low copy BioBrick plasmids are available with the I52002 insert. See I52002 Physical DNA for the current list of plasmids that are available in this form.

Usage and Biology

For more information on how to use this BioBrick part, visit the Featured Parts:Cell Death page.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 435
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 323