Part:BBa_C2001

Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)

Variant of Zif23-GCN4 (see Part:BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added.

Usage and Biology

Protein Database (PDB) Entry: [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1llm link]

Wolfe et al., "Structure of a Designed Dimeric Zinc Finger Protein Bound to DNA" [http://pubs.acs.org/cgi-bin/sample.cgi/bichaw/2003/42/i46/html/bi034830b.html link] Biochemistry 42:13401

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 319
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 79
  
• 1000
  COMPATIBLE WITH RFC[1000]