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  • Part:BBa K2201004
    ===Shake Flask Cultivation=== ...-Bielefeld-CeBiTec--Parts Shakeflask.jpeg|500px|thumb|right|<b>Figure (2): Shake flask cultivation of all <i>Pt</i>NTT2 variants. </b><br> <i>E.&nbsp;coli</
    64 KB (10,011 words) - 08:18, 6 December 2017
  • Part:BBa K3633004
    ...100 mg/L L-Tryptophan in one shake flask and no substrate in another. Both shake flasks successfully produced indigo, and the one with the substrate produce ...100 mg/L L-Tryptophan in one shake flask and no substrate in another. Both shake flasks successfully produced indigo.
    8 KB (1,242 words) - 07:06, 4 October 2021
  • Part:BBa K771305
    **Transfer a fresh transformed colony to 5ml LB liquid medium and shake at 37℃ overnight(12h). ...d broth to 50ml LB medium(volume ratio is 3% and loaded liquid is 20%) and shake until the OD600 reaches 0.6 or so. Add inducer of appropriate concentration
    14 KB (2,052 words) - 01:59, 30 September 2012
  • Part:BBa K771306
    **Transfer a fresh transformed colony to 5ml LB liquid medium and shake at 37℃ overnight(12h). ...d broth to 50ml LB medium(volume ratio is 3% and loaded liquid is 20%) and shake until the OD600 reaches 0.6 or so. Add inducer of appropriate concentration
    13 KB (2,039 words) - 02:02, 30 September 2012
  • Part:BBa K771307
    **Transfer a fresh transformed colony to 5ml LB liquid medium and shake at 37℃ overnight(12h). ...d broth to 50ml LB medium(volume ratio is 3% and loaded liquid is 20%) and shake until the OD600 reaches 0.6 or so. Add inducer of appropriate concentration
    13 KB (2,034 words) - 02:02, 30 September 2012
  • Part:BBa K771308
    **Transfer a fresh transformed colony to 5ml LB liquid medium and shake at 37℃ overnight(12h). ...d broth to 50ml LB medium(volume ratio is 3% and loaded liquid is 20%) and shake until the OD600 reaches 0.6 or so. Add inducer of appropriate concentration
    13 KB (2,034 words) - 02:03, 30 September 2012
  • Part:BBa I0500
    ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to grow at 37℃ for 5~7 hours until the bacteria solution becomes tur ...olution mentioned in step 1 to dilute the bacteria by the ratio of 1:1000. Shake the solution to grow the bacteria at 37℃ overnight.<br>
    27 KB (4,214 words) - 13:09, 1 October 2024
  • Part:BBa J23100
    ...0nm (for cell growth) every 30 seconds for 2 hours in a microplate reader. Shake between readings. Because plateau was reached within the first 30 minutes o ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to grow at 37℃ for 5~7 hours until the bacteria solution becomes tur
    62 KB (9,625 words) - 06:39, 2 October 2024
  • Part:BBa J23109
    ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to to dilute the bacteria by the ratio of 1:1000. Shake the solution to grow the bacteria at 37℃ overnight.<br>
    17 KB (2,644 words) - 07:00, 2 October 2024
  • Part:BBa J23107
    ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to grow at 37℃ for 5~7 hours until the bacteria solution becomes tur ...olution mentioned in step 1 to dilute the bacteria by the ratio of 1:1000. Shake the solution to grow the bacteria at 37℃ overnight.<br>
    10 KB (1,606 words) - 17:30, 27 October 2020
  • Part:BBa K1702005
    <span style="font-size:0.8em">Growth of E. coli DH5alpha cells in shake flasks with this construct over 24 hours as compared to a lac promoter cont <span style="font-size:0.8em">Growth of E. coli DH5alpha cells in shake flasks with this construct over 72 hours as compared to a lac promoter cont
    3 KB (455 words) - 04:45, 25 September 2015
  • Part:BBa K3909024
    ===2. Shake flask cultivations for producing γ-linolenic acid=== The procedure of genomic integration of shake flask cultivations was described above. The result has been showed in Figur
    7 KB (1,004 words) - 11:34, 16 October 2021
  • Part:BBa R0062:Experience
    ...% Sucrose solution and not adding any sucrose solution to cultivate in 37% shake flask , and detect the change of OD value and fluorescence value between 12 ...B in 100 mL LB medium with gradient osmotic pressure stress and culture in shake-flask in 37°C.Calculate RFU of each time point ,and we get a surprising re
    44 KB (6,885 words) - 03:38, 2 November 2017
  • Part:BBa K2306010
    ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to grow at 37℃ for 5~7 hours until the bacteria solution becomes tur ...olution mentioned in step 1 to dilute the bacteria by the ratio of 1:1000. Shake the solution to grow the bacteria at 37℃ overnight.<br>
    26 KB (4,071 words) - 17:58, 27 October 2020
  • Part:BBa K3046001
    ...croscope, a microbioreactor (BioLector, m2p-labs) for microtiter scale, in shake flasks for medium scale and in a 1 liter bioreactor for large scale. In the medium scale, cultures were grown in 200 mL minimal media in 500 mL shake flasks with baffles at 30 °C and 250 rpm. Here samples were taken with reg
    6 KB (944 words) - 03:13, 14 December 2019
  • Part:BBa K3046002
    ...croscope, a microbioreactor (BioLector, m2p-labs) for microtiter scale, in shake flasks for medium scale and in a 1 liter bioreactor for large scale. In the medium scale, cultures were grown in 200 mL minimal media in 500 mL shake flasks with baffles at 30 °C and 250 rpm. Here samples were taken with reg
    6 KB (915 words) - 03:14, 14 December 2019
  • Part:BBa K3251001
    Adding 5mL LB and 5μL AMP respectively to three shake tubes, a, b, c, then these tubes were overturned for 20 times. <br> Adding 5mL LB, 5μLAMP and 5μL, 0.1M IPTG to three shake tubes D, E, F respectively. <br>
    3 KB (481 words) - 02:21, 22 October 2021
  • Part:BBa K3457009
    ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to grow at 37℃ for 5~7 hours until the bacteria solution becomes tur ...olution mentioned in step 1 to dilute the bacteria by the ratio of 1:1000. Shake the solution to grow the bacteria at 37℃ overnight.<br>
    8 KB (1,308 words) - 17:50, 27 October 2020
  • Part:BBa K3457011
    ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to grow at 37℃ for 5~7 hours until the bacteria solution becomes tur ...olution mentioned in step 1 to dilute the bacteria by the ratio of 1:1000. Shake the solution to grow the bacteria at 37℃ overnight.<br>
    8 KB (1,201 words) - 00:38, 27 October 2020
  • Part:BBa K3457012
    ...good condition and put them into 500 μL LB medium containing antibiotics. Shake them to grow at 37℃ for 5~7 hours until the bacteria solution becomes tur ...olution mentioned in step 1 to dilute the bacteria by the ratio of 1:1000. Shake the solution to grow the bacteria at 37℃ overnight.<br>
    8 KB (1,267 words) - 00:38, 27 October 2020

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