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  • Part:BBa K1763444
    ...ge domains proves to be highly difficult, especially when it comes to gene assembly and oligo synthesis. ...s work suggests that the NTD-CTD structures are critical features of fiber assembly, and may allow for the delivery of internally inserted domains into the sil
    34 KB (4,992 words) - 03:39, 19 September 2015
  • Part:BBa K4170045
    ...was developed in accordance with standardization, modularity and standard assembly rules aiming to enhance the CRISPR/crRNA applicability in synthetic biology According to literature references, the crRNA stability is significantly enhanced when it binds to Cas13a enzy
    12 KB (1,886 words) - 11:51, 10 October 2022
  • Part:BBa K4170048
    ...was developed in accordance with standardization, modularity and standard assembly rules aiming to enhance the CRISPR/crRNA applicability in synthetic biology According to literature references, the crRNA stability is significantly enhanced when it binds to Cas13a enzy
    12 KB (1,865 words) - 11:53, 10 October 2022
  • Part:BBa K4170046
    ...was developed in accordance with standardization, modularity and standard assembly rules aiming to enhance the CRISPR/crRNA applicability in synthetic biology According to literature references, the crRNA stability is significantly enhanced when it binds to Cas13a enzy
    12 KB (1,883 words) - 11:52, 10 October 2022
  • Part:BBa K4170047
    ...was developed in accordance with standardization, modularity and standard assembly rules aiming to enhance the CRISPR/crRNA applicability in synthetic biology According to literature references, the crRNA stability is significantly enhanced when it binds to Cas13a enzy
    12 KB (1,876 words) - 11:52, 10 October 2022
  • Part:BBa K4170049
    ...was developed in accordance with standardization, modularity and standard assembly rules aiming to enhance the CRISPR/crRNA applicability in synthetic biology According to literature references, the crRNA stability is significantly enhanced when it binds to Cas13a enzy
    12 KB (1,875 words) - 11:53, 10 October 2022
  • Part:BBa K4170050
    ...was developed in accordance with standardization, modularity and standard assembly rules aiming to enhance the CRISPR/crRNA applicability in synthetic biology According to literature references, the crRNA stability is significantly enhanced when it binds to Cas13a enzy
    12 KB (1,892 words) - 11:54, 10 October 2022
  • Part:BBa K2201004
    ...> used an N-terminal truncated version of <i>Pt</i>NTT2, lacking the first 65 amino acids, since they observed some kind of toxicity resulting from the n ...ersions with new signal peptides were constructed using primers and Gibson Assembly.
    64 KB (10,011 words) - 08:18, 6 December 2017
  • Part:BBa K3410001
    ...y (sample “P_K”) amplified by epPCR using the kit from JenaBioScience [65] and as a reference the estradiol nanobody amplificated by a OneTaq® High ...tion of the nanobodiies, the IMPACT kit will be used: Using Through Gibson assembly, the nanobody sequence of our affinity maturated nanobodies is cloned into
    56 KB (8,191 words) - 03:28, 28 October 2020
  • Part:BBa K3410002
    ...y (sample “P_K”) amplified by epPCR using the kit from JenaBioScience [65] and as a reference the estradiol nanobody amplificated by a OneTaq® High ...tion of the nanobodiies, the IMPACT kit will be used: Using Through Gibson assembly, the nanobody sequence of our affinity maturated nanobodies is cloned into
    57 KB (8,247 words) - 03:32, 28 October 2020
  • Part:BBa K3410004
    ...y (sample “P_K”) amplified by epPCR using the kit from JenaBioScience [65] and as a reference the estradiol nanobody amplificated by a OneTaq® High ...tion of the nanobodiies, the IMPACT kit will be used: Using Through Gibson assembly, the nanobody sequence of our affinity maturated nanobodies is cloned into
    57 KB (8,249 words) - 03:33, 28 October 2020
  • Part:BBa J45014
    ...he ligation of the protein of interest into the pET-19b plasmid via Gibson Assembly. ...nd of the ATF1 sequence to allow for ligation to pET19b backbone by Gibson Assembly.
    20 KB (2,977 words) - 10:34, 12 October 2022
  • Part:BBa K4927058
    ...e high-efficiency CE7-IM7 protein reaction pair, which can be used for the assembly of artificial complexes in multi-enzyme cascade reaction systems. ...chieved through organometallic frameworks, fusion protein expression, self-assembly microstructures, and artificial biological scaffolds (Figure 1) [7-9]. Amon
    21 KB (3,106 words) - 07:08, 9 October 2023
  • Part:BBa K1150034
    |'''RFC standard''' Multiple target sites can be combined on one plasmid by using the BioBrick standard and enables targeting of multiple loci at once with a small amount of plasm
    6 KB (949 words) - 02:26, 29 October 2013
  • Part:BBa E1010
    <b>References</b> ...sed a small amount of the sample into 12 PCR tubes, and a gradient test at 65-95℃ was conducted in a PCR machine for 10 minutes.<br>
    66 KB (8,421 words) - 06:22, 12 October 2022
  • Part:BBa P1016
    |width='65%'| ...Positive selection markers prevent one of the most common problems during assembly of BioBrick parts: contamination of the ligation reaction with uncut plasmi
    4 KB (536 words) - 18:56, 9 July 2015
  • Part:BBa K2082251
    ...ck-in strain was about 3000 bp in size. With the 1 kb ladder as the length standard, it is seen that the colonies 2, 6, 7, 9 and 13 have the expected result.</ Assembly of the guide RNA in pTarget:
    13 KB (2,163 words) - 17:58, 21 October 2016
  • Part:BBa K2934000
    ...hed to the vector per the sequencing result, and Gox1 protein was found at 65 Kda by SDS-PAGE</figcaption> ...ell plate diagram of the GSH standard samples. Fig. 3B is the GSH activity standard curve. Fig. 3C shows the 96 well plate diagram of GSH experimental samples.
    11 KB (1,571 words) - 09:44, 10 October 2023
  • Part:BBa K4130000
    The EibD biobrick was assembled into the pSB1C3 vector by 3A assembly and cloned into E. coli DH5a for storage and E. coli BL21 for expression ex ...calculated for each time point. The error ribbon on the graph represents 1 standard deviation from the mean (n=3). The addition of the anti-asparagine antibody
    28 KB (4,120 words) - 23:56, 13 October 2022
  • Part:BBa K2005050
    ...d to minimize its susceptibility to oxidative DNA mutation. Compared with standard mCherry, this gene is therefore more stable and less likely to evolve out o ...ced it at similar levels. For this sequence, our algorithm could eliminate 65% of poly-guanine motifs and an overall reduction in oxidizable guanines.
    4 KB (555 words) - 06:38, 29 October 2017

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