User contributions
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- 20:22, 21 October 2019 (diff | hist) . . (-52) . . Part:BBa K2965021
- 20:16, 21 October 2019 (diff | hist) . . (0) . . Part:BBa K2965021
- 20:12, 21 October 2019 (diff | hist) . . (+46) . . Part:BBa K2965021
- 20:03, 21 October 2019 (diff | hist) . . (+16) . . Part:BBa K2965021
- 16:25, 21 October 2019 (diff | hist) . . (+2) . . Part:BBa K2965021
- 16:19, 21 October 2019 (diff | hist) . . (+18) . . Part:BBa K2965021
- 16:15, 21 October 2019 (diff | hist) . . (+437) . . Part:BBa K2965021
- 15:43, 21 October 2019 (diff | hist) . . (0) . . File:BBa K2965021-Table 1. AsCas12a concentration..png (Alexiss uploaded a new version of File:BBa K2965021-Table 1. AsCas12a concentration..png) (current)
- 15:38, 21 October 2019 (diff | hist) . . (+367) . . Part:BBa K2965021
- 14:36, 21 October 2019 (diff | hist) . . (+28) . . Part:BBa K2965021 (→Result)
- 14:26, 21 October 2019 (diff | hist) . . (+2) . . Part:BBa R0011
- 09:06, 21 October 2019 (diff | hist) . . (-10) . . Part:BBa R0011
- 09:03, 21 October 2019 (diff | hist) . . (-10) . . Part:BBa K2965037
- 09:02, 21 October 2019 (diff | hist) . . (+1) . . Part:BBa K2965037
- 09:01, 21 October 2019 (diff | hist) . . (-48) . . Part:BBa K2965037
- 08:58, 21 October 2019 (diff | hist) . . (+1) . . Part:BBa K2965037
- 08:57, 21 October 2019 (diff | hist) . . (-26) . . Part:BBa K2965037
- 08:57, 21 October 2019 (diff | hist) . . (+4,983) . . Part:BBa K2965037
- 08:51, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965037-Figure 4. Quantitatively compared FnCas12a’s activity when using Fn-crRNA-HPV16-1 or 2..PNG (current)
- 08:47, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965037-Figure 3. The comparison between different Cas12a and different crRNA targeting different sequences..PNG (current)
- 08:45, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965037-Figure 2. Doing DNA reporter cleavage assay with gradient ion concentration..PNG (current)
- 08:41, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965037-Figure 1. 500bp from HPV16 L1 gene..PNG (current)
- 08:33, 21 October 2019 (diff | hist) . . (+1) . . Part:BBa R0011
- 08:32, 21 October 2019 (diff | hist) . . (0) . . Part:BBa R0011
- 08:23, 21 October 2019 (diff | hist) . . (0) . . File:BBa K2965021-Table 1. AsCas12a concentration..png (Alexiss uploaded a new version of File:BBa K2965021-Table 1. AsCas12a concentration..png)
- 08:22, 21 October 2019 (diff | hist) . . (+79) . . Part:BBa K2965021
- 08:21, 21 October 2019 (diff | hist) . . (-116) . . Part:BBa K2965021
- 08:18, 21 October 2019 (diff | hist) . . (+118) . . Part:BBa K2965021
- 08:15, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965021-Table 1. AsCas12a concentration..png
- 08:09, 21 October 2019 (diff | hist) . . (-10) . . Part:BBa K2965021
- 08:08, 21 October 2019 (diff | hist) . . (+1,101) . . Part:BBa K2965021
- 08:06, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965021-Figure 5. AsCas12a activity assay..png (current)
- 08:03, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965021-Figure 4. BSA standard curve..png (current)
- 07:56, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965021-Figure 3. AsCas12a WB..png (current)
- 07:53, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965021-Figure 2. AsCas12a SDS-PAGE..png (current)
- 07:52, 21 October 2019 (diff | hist) . . (0) . . Part:BBa K2965021
- 07:51, 21 October 2019 (diff | hist) . . (+7) . . Part:BBa K2965021
- 07:50, 21 October 2019 (diff | hist) . . (-1) . . Part:BBa K2965021
- 07:49, 21 October 2019 (diff | hist) . . (-49) . . Part:BBa K2965021
- 07:47, 21 October 2019 (diff | hist) . . (+1,372) . . Part:BBa K2965021
- 07:45, 21 October 2019 (diff | hist) . . (0) . . N File:BBa K2965021-Figure 1. AsCas12a colony PCR..png (current)
- 06:44, 21 October 2019 (diff | hist) . . (0) . . File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png (Alexiss uploaded a new version of [[File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png]) (current)
- 06:40, 21 October 2019 (diff | hist) . . (0) . . File:2019 ZJU-China Characterization Figure 3.The relative normalized expression level of EGFP of E.coli BL21 (DE3) under the conditions of IPTG concentration of 0 mM, 0.02mM, 0.08 mM, 0.3mM and 0.5 mM at 25℃..png (Alexiss uploaded a new version of File:2019 ZJU-China Characterization Figure 3.The relative normalized expression level of EGFP of E.coli BL21 (DE3) under the conditions of IPTG concentration of 0 mM, 0.02mM, 0.08 mM, 0.3mM and 0.5 mM at 25℃..png) (current)
- 06:34, 21 October 2019 (diff | hist) . . (-6) . . Part:BBa R0011
- 06:32, 21 October 2019 (diff | hist) . . (0) . . File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png (Alexiss uploaded a new version of [[File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png])
- 06:15, 21 October 2019 (diff | hist) . . (0) . . File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png (Alexiss uploaded a new version of [[File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png])
- 06:13, 21 October 2019 (diff | hist) . . (0) . . File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png (Alexiss uploaded a new version of [[File:2019 ZJU-China Characterization Figure 2.The trend of green fluorescence intensity of E.coli BL21 (DE3) under the conditions of IPTG of 0 mM, 0.05 mM, 0.1 mM and 0.5 mM at 18℃, 25℃ and 37℃, respectively..png])
- 05:59, 21 October 2019 (diff | hist) . . (0) . . File:2019 ZJU-China Characterization Figure 3.The relative normalized expression level of EGFP of E.coli BL21 (DE3) under the conditions of IPTG concentration of 0 mM, 0.02mM, 0.08 mM, 0.3mM and 0.5 mM at 25℃..png (Alexiss uploaded a new version of File:2019 ZJU-China Characterization Figure 3.The relative normalized expression level of EGFP of E.coli BL21 (DE3) under the conditions of IPTG concentration of 0 mM, 0.02mM, 0.08 mM, 0.3mM and 0.5 mM at 25℃..png)
- 05:58, 21 October 2019 (diff | hist) . . (0) . . File:2019 ZJU-China Characterization Figure 3.The relative normalized expression level of EGFP of E.coli BL21 (DE3) under the conditions of IPTG concentration of 0 mM, 0.02mM, 0.08 mM, 0.3mM and 0.5 mM at 25℃..png (Alexiss uploaded a new version of File:2019 ZJU-China Characterization Figure 3.The relative normalized expression level of EGFP of E.coli BL21 (DE3) under the conditions of IPTG concentration of 0 mM, 0.02mM, 0.08 mM, 0.3mM and 0.5 mM at 25℃..png)
- 05:55, 21 October 2019 (diff | hist) . . (+1,038) . . Part:BBa R0011
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