Protein coding sequences/Transcriptional regulators

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Transcriptional regulators either activate or repress transcription from cognate promoters.

Activators

Activators typically bind nearby to transcriptional promoters and recruit RNA polymerase to initiate transcription.

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Name	Protein	Description	Tag	Direction	Uniprot	KEGG	Operator	Ligand	Length
BBa_C0079	lasR-LVA	lasR activator from P. aeruginosa PAO1(+LVA)	LVA	Forward	P25084				781
BBa_C0077	cinR	cinR activator from Rhizobium leguminosarum (+LVA)	LVA	Forward	~ Q84HT2				787
BBa_C0179	lasR	lasR activator from P. aeruginosa PAO1(no LVA)	None	Forward	P25084				723
BBa_J07009	ToxR	toxicity-gene activator from Vibrio cholerae	None	Forward	P15795				630
BBa_K118001		appY coding sequence encoding a DNA-binding transcriptional activator							753
BBa_K082006		LuxR-G2F							753
BBa_S04301	lasR-LVA	C0079:B0015	LVA	Forward	P25084				918
BBa_K266002	lasR-LVA	LasR + Term	LVA	Forward	P25084				918
BBa_K389002		Mutated virG							740
BBa_K352001	transcription activator	CooA from Rhodospirillum rubrum				avn:Avin_47010			669
BBa_K783054	lasR-LVA	This is a MoClo converted version of BBa_C0079	LVA	Forward	P25084				756
BBa_K1031410		HcaR-Terminator							1046
BBa_K1031444		J23114-HcaR							952
BBa_K1315005		The clp gene from Xanthomonas campestris		Forward					696
BBa_K1486056		CxpR & Split IFP1.4 [Cterm + Cterm][2]		Forward					3742
BBa_K137113		rcsA							624
BBa_K131022		LuxO D47E, Vibrio harveyi							1362
BBa_K131023		LuxO D47A, Vibrio harveyi							1362
BBa_K748000		AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.							630
BBa_K1031911	XylS	XylS-Terminator							1103
BBa_K1031100		CapR-Terminator							1827
BBa_K1031111		J23109-CapR-Terminator							1888
BBa_K1031112		J23113-CapR-Terminator							1888
BBa_K1031441		J23106-HcaR							952
BBa_K1031442		J23109-HcaR							952
BBa_K1031443		J23113-HcaR							952
BBa_K1031912		J23105-XylS-Terminator							1162
BBa_K1031913		J23109-XylS-Terminator							1162
BBa_K1031916		J23114-XylS-Terminator							1162
BBa_K1031311		J23114-HbpR-Terminator							1910
BBa_K1031917		J23117-XylS-Terminator							1163
BBa_K1031211		Pr-DmpR							1766
BBa_K1031017		J23114-B0034-RiboJ-φR73δ							381
BBa_K1031018		Psal-B0034-RiboJ-φR73δ							480
BBa_K1031019		NahR-Psal-B0034-RiboJ-φR73δ							1615
BBa_K1486001		Arabinose promoter + CpxR		Forward					1924
BBa_K1467205		AvrBS3 TAL Effector - Induces BS3							3505
BBa_K1639002		GadE							546
BBa_K2036000		CII bacteriophage lambda trascriptional activator							312
BBa_K1946000		ChnR							942
BBa_K1946005		XylR							1701
BBa_K2023010	XylR	XylR gene with His tag	His						1728
BBa_K2549036		VP64-ZF21.16N-CfaN							612
BBa_K5466010		LexA-VP16							897
BBa_K294205		This is a coding sequence of heat shock protein from E.coli							402
BBa_J120015		Coding sequence for PAX3, a regulatory transcription factor in H. sapiens							645
BBa_M36888		Sequence for sigma 32 transcription factor							861
BBa_K1486000		CpxR coding region		Forward					702
BBa_K1709004		LuxR-E	E-tag	Forward					801
BBa_K1895007		Sequence for sigma 32 transcription factor							861
BBa_K2317006		DmpR							1692
BBa_K2549045		linker between GAL4 DNA binding domain and VP64 transcription activator							36
BBa_K2549057		VP64							150
BBa_K3205002		AreR_Acinetobacter sp. Strain ADP1							1803
BBa_K3206004	GcvA transcription factor	GcvA gene							918
BBa_K3350857		yhaJ (DNA-binding transcriptional activator)							897
BBa_K3350858		yhaJ1st (DNA-binding transcriptional activator)							897

Repressors

Repressors bind to transcriptional promoters and sterically hinder transcriptional initiation by RNA polymerase

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Name	Protein	Description	Tag	Direction	Uniprot	KEGG	Operator	Ligand	Length
BBa_C0012	LacI	lacI repressor from E. coli (+LVA)	LVA	Forward	P03023				1153
BBa_C0040	TetR	tetracycline repressor from transposon Tn10 (+LVA)	LVA	Forward	P04483				685
BBa_C0050	CI HK022	cI repressor from phage HK022 (+LVA?)	LVA	Forward	P18680				769
BBa_C0051	CI lambda	cI repressor from E. coli phage lambda (+LVA)	LVA	Forward	P03034				775
BBa_C0052	CI 434-LVA	cI repressor from phage 434 (+LVA)	LVA	Forward	P16117				694
BBa_C0053	C2 P22	c2 repressor from Salmonella phage P22 (+LVA)	LVA	Forward	P69202				712
BBa_C0073	mnt-weak	mnt repressor (weak) from Salmonella phage P22 (+LVA)	LVA	Forward	P03049				313
BBa_C0074	penI	penI repressor from Bacillus licheniformis (+LVA)	LVA	Forward	P06555				448
BBa_C0075	cI TP901	TP901 cI repressor from phage TP901-1 (+LVA)	LVA	Forward	none				604
BBa_C0072	mnt	mnt repressor (strong) from Salmonella phage P22 (+LVA)	LVA	Forward	P03049				313
BBa_C2001	Zif23-GCN4	Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)	LVA	Forward	P03069				325
BBa_C0056	CI 434	cI repressor from phage 434 (no LVA)	None	Forward	P16117				636
BBa_J06501	LacI-mut2	LacI repressor (temperature-sensitive mut 265) (+LVA)	LVA	Forward	~ P03023				1153
BBa_C2006		MalE.FactorXa.Zif268-GCN4							1428
BBa_I715032		lacIq reverse							1128
BBa_I732100		LacI							1086
BBa_I732101		LRLa							1086
BBa_I732105		ARL2A0101							1086
BBa_I732106		ARL2A0102							1086
BBa_I732107		ARL2A0103							1086
BBa_I732110		ARL2A0203							1086
BBa_I732112		ARL2A0301							1086
BBa_I732115		ARL4A0604							1086
BBa_K091001		LsrR gene		Forward					954
BBa_K143033		LacI (Lva-, N-terminal deletion) regulatory protein							1086
BBa_K082004	LacI	LacI- wild type							1083
BBa_K950001		yeast rox1							1225
BBa_K950009		yeast mig1							1515
BBa_K864201	LexA	LexA SOS response repressor		Forward					612
BBa_K864202	MarR(G95S)	MarR(G95S) super-repressor		Forward					438
BBa_K864203	LexA3	LexA3 super-repressor		Forward					612
BBa_K1031510		HpaR-Terminator							602
BBa_K1509000		Coding for the trans-acting regulator SmtB							369
BBa_K1682011		NsrR							426
BBa_K2056001		NsrR - NO(x) sensitive pYear, pNirk repressor protein							426
BBa_J06500	LacI-mut1	LacI repressor (temperature-sensitive mut 241) (+LVA)	LVA	Forward	~ P03023				1153
BBa_K091121		LacI wild-type gene							1083
BBa_K091122		LacI_I12 protein							1083
BBa_K142000		lacI IS mutant (IPTG unresponsive) R197A							1128
BBa_K142001		lacI IS mutant (IPTG unresponsive) R197F							1128
BBa_K142002		lacI IS mutant (IPTG unresponsive) T276A							1128
BBa_K142003		lacI IS mutant (IPTG unresponsive) T276F							1128
BBa_K106666		Lac Repressor, AarI AB part							1104
BBa_K106667		Lac Repressor, AarI BD part							1107
BBa_K142004		lacI IS mutant (IPTG unresponsive) R197A T276A							1128
BBa_K106668		Tet Repressor, AarI AB part							618
BBa_K106669		Tet Repressor, AarI BD part							621
BBa_K142005		lacI IS mutant (IPTG unresponsive) R197A T276F							1128
BBa_K142006		lacI IS mutant (IPTG unresponsive) R197F T276A							1128
BBa_K142007		lacI IS mutant (IPTG unresponsive) R197F T276F							1128
BBa_K082005	LacI	LacI-Mutant							1083
BBa_K518003		LexA - a repressor for LexA-regulated SOS promoters -							750
BBa_K864200	MarR	MarR native E. coli repressor		Forward					438
BBa_K1122666		Ferric uptake repressor							450
BBa_K1031710		PaaX-Terminator							1106
BBa_K1031742		J23109-PaaX							1012
BBa_K1031743		J23113-PaaX							1012
BBa_K1031744		J23114-PaaX							1012
BBa_K2549037		KRAB-ZF21.16N-CfaN							687
BBa_K4056003		HA-tag MphR							615
BBa_K3848009		LLDR							782
BBa_K4133006		Repressor protein CtsR_Lp (Lactobacillus plantarum WCFS1) for Lactobacillus casei							468
BBa_K4133009		Repressor protein CtsR_Lc (Lactococcus lactis) for Lactobacillus casei							456
BBa_K2549055		KRAB							225
BBa_K3093000		chbR N238S Y30C							843
BBa_K3505003	LacI	LacI-Lac repressor GB compatible with B3-B5							1088

Multiple

Some transcriptional regulators serve as either an activator or a repressor depending on where it binds and cellular conditions

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Name	Protein	Description	Tag	Direction	Uniprot	KEGG	Operator	Ligand	Length
BBa_C0062	LuxR	luxR repressor/activator, (no LVA?)	None	Forward	P12746				781
BBa_C0071	rhlR-LVA	rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)	LVA	Forward	P54292				787
BBa_C0080	araC	araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)	LVA	Forward	P0A9E0				940
BBa_C0171	rhIR	rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)	None	Forward	P54292				729
BBa_K108021		Fis							297
BBa_K2656016		LuxR TF Coding Sequence							757