Protein coding sequences/Transcriptional regulators

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Transcriptional regulators either activate or repress transcription from cognate promoters.

Activators

PositivePromoter.png Activators typically bind nearby to transcriptional promoters and recruit RNA polymerase to initiate transcription.


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NameProteinDescriptionTagDirectionUniprotKEGGOperatorLigandLength
BBa_C0079lasR-LVAlasR activator from P. aeruginosa PAO1(+LVA)LVAForwardP25084   781
BBa_C0077cinRcinR activator from Rhizobium leguminosarum (+LVA)LVAForward~ Q84HT2   787
BBa_C0179lasRlasR activator from P. aeruginosa PAO1(no LVA)NoneForwardP25084   723
BBa_J07009ToxR toxicity-gene activator from Vibrio choleraeNoneForwardP15795   630
BBa_K118001 appY coding sequence encoding a DNA-binding transcriptional activator    753
BBa_K082006 LuxR-G2F    753
BBa_S04301lasR-LVAC0079:B0015LVAForwardP25084   918
BBa_K266002lasR-LVALasR + TermLVAForwardP25084   918
BBa_K389002 Mutated virG    740
BBa_K352001transcription activatorCooA from Rhodospirillum rubrum avn:Avin_47010  669
BBa_K783054lasR-LVAThis is a MoClo converted version of BBa_C0079LVAForwardP25084   756
BBa_K1031410 HcaR-Terminator    1046
BBa_K1031444 J23114-HcaR    952
BBa_K1315005 The clp gene from Xanthomonas campestrisForward    696
BBa_K1486056 CxpR & Split IFP1.4 [Cterm + Cterm][2]Forward    3742
BBa_K137113 rcsA    624
BBa_K131022 LuxO D47E, Vibrio harveyi    1362
BBa_K131023 LuxO D47A, Vibrio harveyi    1362
BBa_K748000 AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.    630
BBa_K1031911XylS XylS-Terminator    1103
BBa_K1031100 CapR-Terminator    1827
BBa_K1031111 J23109-CapR-Terminator    1888
BBa_K1031112 J23113-CapR-Terminator    1888
BBa_K1031441 J23106-HcaR    952
BBa_K1031442 J23109-HcaR    952
BBa_K1031443 J23113-HcaR    952
BBa_K1031912 J23105-XylS-Terminator    1162
BBa_K1031913 J23109-XylS-Terminator    1162
BBa_K1031916 J23114-XylS-Terminator    1162
BBa_K1031311 J23114-HbpR-Terminator    1910
BBa_K1031917 J23117-XylS-Terminator    1163
BBa_K1031211 Pr-DmpR    1766
BBa_K1031017 J23114-B0034-RiboJ-φR73δ    381
BBa_K1031018 Psal-B0034-RiboJ-φR73δ    480
BBa_K1031019 NahR-Psal-B0034-RiboJ-φR73δ    1615
BBa_K1486001 Arabinose promoter + CpxRForward    1924
BBa_K1467205 AvrBS3 TAL Effector - Induces BS3    3505
BBa_K1639002 GadE    546
BBa_K2036000 CII bacteriophage lambda trascriptional activator    312
BBa_K1946000 ChnR    942
BBa_K1946005 XylR    1701
BBa_K2023010XylRXylR gene with His tagHis    1728
BBa_K2549036 VP64-ZF21.16N-CfaN     612
BBa_K294205 This is a coding sequence of heat shock protein from E.coli    402
BBa_J120015 Coding sequence for PAX3, a regulatory transcription factor in H. sapiens    645
BBa_M36888 Sequence for sigma 32 transcription factor     861
BBa_K1486000 CpxR coding regionForward    702
BBa_K1709004 LuxR-EE-tagForward    801
BBa_K1895007 Sequence for sigma 32 transcription factor     861
BBa_K2317006 DmpR    1692
BBa_K2549045 linker between GAL4 DNA binding domain and VP64 transcription activator    36
BBa_K2549057 VP64    150
BBa_K3205002 AreR_Acinetobacter sp. Strain ADP1    1803
BBa_K3206004GcvA transcription factorGcvA gene     918
BBa_K3350857 yhaJ (DNA-binding transcriptional activator)    897
BBa_K3350858 yhaJ1st (DNA-binding transcriptional activator)    897


Repressors

NegativePromoter.png Repressors bind to transcriptional promoters and sterically hinder transcriptional initiation by RNA polymerase


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NameProteinDescriptionTagDirectionUniprotKEGGOperatorLigandLength
BBa_C0012LacIlacI repressor from E. coli (+LVA)LVAForwardP03023   1153
BBa_C0040TetRtetracycline repressor from transposon Tn10 (+LVA)LVAForwardP04483   685
BBa_C0050CI HK022 cI repressor from phage HK022 (+LVA?)LVAForward P18680   769
BBa_C0051CI lambdacI repressor from E. coli phage lambda (+LVA) LVAForwardP03034   775
BBa_C0052CI 434-LVAcI repressor from phage 434 (+LVA)LVAForwardP16117   694
BBa_C0053C2 P22c2 repressor from Salmonella phage P22 (+LVA)LVAForwardP69202   712
BBa_C0073mnt-weakmnt repressor (weak) from Salmonella phage P22 (+LVA)LVAForwardP03049   313
BBa_C0074penIpenI repressor from Bacillus licheniformis (+LVA)LVAForwardP06555   448
BBa_C0075cI TP901TP901 cI repressor from phage TP901-1 (+LVA)LVAForwardnone   604
BBa_C0072mntmnt repressor (strong) from Salmonella phage P22 (+LVA)LVAForwardP03049   313
BBa_C2001Zif23-GCN4Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)LVAForwardP03069   325
BBa_C0056CI 434 cI repressor from phage 434 (no LVA)NoneForwardP16117   636
BBa_J06501LacI-mut2LacI repressor (temperature-sensitive mut 265) (+LVA)LVAForward~ P03023   1153
BBa_C2006 MalE.FactorXa.Zif268-GCN4    1428
BBa_I715032 lacIq reverse    1128
BBa_I732100 LacI    1086
BBa_I732101 LRLa    1086
BBa_I732105 ARL2A0101    1086
BBa_I732106 ARL2A0102    1086
BBa_I732107 ARL2A0103    1086
BBa_I732110 ARL2A0203    1086
BBa_I732112 ARL2A0301    1086
BBa_I732115 ARL4A0604    1086
BBa_K091001 LsrR geneForward    954
BBa_K143033 LacI (Lva-, N-terminal deletion) regulatory protein    1086
BBa_K082004LacILacI- wild type    1083
BBa_K950001 yeast rox1    1225
BBa_K950009 yeast mig1    1515
BBa_K864201LexALexA SOS response repressorForward    612
BBa_K864202MarR(G95S)MarR(G95S) super-repressorForward    438
BBa_K864203LexA3LexA3 super-repressorForward    612
BBa_K1031510 HpaR-Terminator    602
BBa_K1509000 Coding for the trans-acting regulator SmtB    369
BBa_K1682011 NsrR    426
BBa_K2056001 NsrR - NO(x) sensitive pYear, pNirk repressor protein    426
BBa_J06500LacI-mut1LacI repressor (temperature-sensitive mut 241) (+LVA)LVAForward~ P03023   1153
BBa_K091121 LacI wild-type gene    1083
BBa_K091122 LacI_I12 protein    1083
BBa_K142000 lacI IS mutant (IPTG unresponsive) R197A    1128
BBa_K142001 lacI IS mutant (IPTG unresponsive) R197F    1128
BBa_K142002 lacI IS mutant (IPTG unresponsive) T276A    1128
BBa_K142003 lacI IS mutant (IPTG unresponsive) T276F    1128
BBa_K106666 Lac Repressor, AarI AB part    1104
BBa_K106667 Lac Repressor, AarI BD part    1107
BBa_K142004 lacI IS mutant (IPTG unresponsive) R197A T276A    1128
BBa_K106668 Tet Repressor, AarI AB part    618
BBa_K106669 Tet Repressor, AarI BD part    621
BBa_K142005 lacI IS mutant (IPTG unresponsive) R197A T276F     1128
BBa_K142006 lacI IS mutant (IPTG unresponsive) R197F T276A     1128
BBa_K142007 lacI IS mutant (IPTG unresponsive) R197F T276F    1128
BBa_K082005LacILacI-Mutant    1083
BBa_K518003 LexA - a repressor for LexA-regulated SOS promoters -    750
BBa_K864200MarRMarR native E. coli repressorForward    438
BBa_K1122666 Ferric uptake repressor    450
BBa_K1031710 PaaX-Terminator    1106
BBa_K1031742 J23109-PaaX    1012
BBa_K1031743 J23113-PaaX    1012
BBa_K1031744 J23114-PaaX    1012
BBa_K2549037 KRAB-ZF21.16N-CfaN    687
BBa_K4056003 HA-tag MphR    615
BBa_K3848009 LLDR    782
BBa_K4133006 Repressor protein CtsR_Lp (Lactobacillus plantarum WCFS1) for Lactobacillus casei    468
BBa_K4133009 Repressor protein CtsR_Lc (Lactococcus lactis) for Lactobacillus casei    456
BBa_K2549055 KRAB    225
BBa_K3093000 chbR N238S Y30C    843
BBa_K3505003LacILacI-Lac repressor GB compatible with B3-B5    1088


Multiple

MultiplePromoter.png Some transcriptional regulators serve as either an activator or a repressor depending on where it binds and cellular conditions


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NameProteinDescriptionTagDirectionUniprotKEGGOperatorLigandLength
BBa_C0062LuxRluxR repressor/activator, (no LVA?)NoneForwardP12746   781
BBa_C0071rhlR-LVArhlR repressor/activator from P. aeruginosa PA3477 (+LVA)LVAForwardP54292   787
BBa_C0080araCaraC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)LVAForwardP0A9E0   940
BBa_C0171rhIRrhlR repressor/activator from P. aeruginosa PA3477 (no LVA)NoneForwardP54292   729
BBa_K108021 Fis    297
BBa_K2656016 LuxR TF Coding Sequence    757