Protein coding sequences/Selection markers
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Selection markers are protein coding sequences that confer a selective advantage or disadvantage to host chassis. For example, a common type of prokaryotic selection marker is one that confers resistance to a particular antibiotic. Thus, cells that carry the selection marker can grow in media despite the presence of antibiotic. Most plasmids contain antibiotic selection markers so that researchers can ensure that the plasmid is maintained during cell replication and division. (Cells that lose a copy of the plasmid will soon either die or fail to grow in media supplemented with antibiotic.) A second common type of selection marker, often termed a positive selection marker, are those that are toxic to the cell. Positive selection markers are frequently used during cloning to select against cells transformed with the cloning vector and ensure that only cells transformed with a plasmid containing the insert.
| Name | Protein | Description | Tag | Direction | UniProt | KEGG | Length | Status |
|---|---|---|---|---|---|---|---|---|
| BBa_J31006 | TetA(C)-bkw | tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007] | P02981 | 1191 | In stock | |||
| BBa_J31005 | CAT | chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004] | P62577 | none | 660 | In stock | ||
| BBa_J31007 | TetA(C) | tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006] | P02981 | 1191 | In stock | |||
| BBa_K1021003 | bar (bialaphos resistance) | 552 | In stock | |||||
| BBa_K1071001 | ble resistance (eucaryotic) | 375 | In stock | |||||
| BBa_K1323011 | Erythromycin resistance coding sequence | 735 | In stock | |||||
| BBa_K1323012 | Spc - Spectinomycin Resistance Gene Coding Sequence | 783 | In stock | |||||
| BBa_K1323013 | aadD - Kanamycin Resistance Gene Coding Sequence | 726 | In stock | |||||
| BBa_K1313004 | Neomycin Resistance | 786 | In stock | |||||
| BBa_T9150 | PyrF | orotidine 5 | None | Forward | P08244 | eco:b1281; | 741 | It's complicated |
| BBa_J31002 | AadA-bkw | kanamycin resistance backwards (KanB) [cf. BBa_J23012 & BBa_J31003] | P0AG05 | none | 816 | It's complicated | ||
| BBa_J31003 | AadA2 | kanamycin resistance forward (KanF) [cf. BBa_J23012 & BBa_J31002] | P0AG05 | none | 816 | It's complicated | ||
| BBa_J31004 | CAT-bkw | chloramphenicol acetyltransferase (backwards, CmB) [cf. BBa_J31005] | P62577 | none | 660 | It's complicated | ||
| BBa_K143031 | Aad9 Spectinomycin Resistance Gene | 771 | It's complicated | |||||
| BBa_K156011 | aadA (streptomycin 3'-adenyltransferase) | 789 | It's complicated | |||||
| BBa_K389005 | Kanamycin resistance | 819 | It's complicated | |||||
| BBa_K394000 | Resistance Gene for G418 in Yeast | 813 | It's complicated | |||||
| BBa_K678040 | pyrG cassette, for selection of Aspergillus nidulans | 1456 | It's complicated | |||||
| BBa_K678041 | pyrG-DR cassette, for selection of Aspergillus nidulans | 2168 | It's complicated | |||||
| BBa_K678042 | hygromycin cassette for Aspergillus nidulans | 1363 | It's complicated | |||||
| BBa_K678044 | bleR cassette, selection marker for Aspergillus nidulans | 1663 | It's complicated | |||||
| BBa_K678045 | ptrA, selection marker for Aspergillus nidulans | 1899 | It's complicated | |||||
| BBa_K823020 | cat | chloramphenicol acetyltransferase (cat) | 645 | It's complicated | ||||
| BBa_K1207001 | Macrolide-Lincosamide-Streptogramin B Resistance Marker | 735 | It's complicated | |||||
| BBa_T9000 | aacC2 | Forward | 861 | It's complicated | ||||
| BBa_K2799001 | nat1 | 573 | It's complicated | |||||
| BBa_K4056001 | ereB - Erythromycin esterase type II | 1263 | ||||||
| BBa_K4047016 | Gentamicin resistance (GmR) for Synechococcus | 534 | ||||||
| BBa_K4047019 | Kanamycin Resistance for Synechococcus | 816 | ||||||
| BBa_K4235003 | Gentamicin Resistance Gene | 534 | ||||||
| BBa_K4235018 | Ampicillin Resistance Gene | 861 | ||||||
| BBa_K1015002 | streptmycin resisitance gene (can PCR ampicillin resistance gene primer) | 1031 | Not in stock | |||||
| BBa_K1073000 | ampicillin resistance | 864 | Not in stock | |||||
| BBa_K1015009 | Chloramphenicol resistance gene+FRT | 1098 | Not in stock | |||||
| BBa_K1015010 | Ampicillin resistance gene | 983 | Not in stock | |||||
| BBa_K3046028 | PyrG CDS from Aspergillus fumigatus | 906 | Not in stock | |||||
| BBa_K3629011 | Nourseothricin resistance gene | 573 | Not in stock |