Part:BBa_J31005

chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]

When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Chloramphenicol resistance

Chloramphenicol concentrations up to 75μg/ml can be tolerated by bacteria carrying a single copy of the Chloramphenicol acetyltransferase gene, though ideally cells would be grown in medium with a chloramphenicol concentration of less than 30μg/ml.

[http://2008.igem.org/Team:Imperial_College/CAT Results from Imperial iGEM 2008 team, as part of Biofabricator subtilis]