Plasmid

Part:pSB5A5:Design

Designed by: Reshma Shetty   Group: iGEM06_MIT   (2006-06-01)


pSB5A5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5885
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5885
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5891
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5885
    Illegal BglII site found at 3437
    Illegal BglII site found at 5504
    Illegal BglII site found at 6357
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5885
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5885
    Illegal XbaI site found at 5900
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1031
    Illegal NgoMIV site found at 4947
    Illegal AgeI site found at 2700
    Illegal AgeI site found at 3593
    Illegal AgeI site found at 4823
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1846
    Illegal SapI.rc site found at 2843
    Illegal SapI.rc site found at 4053


Design Notes

Source

References