Plasmid_Backbone
pSB2K3

Part:pSB2K3:Design

Designed by: Sri Kosuri   Group: Antiquity   (2004-05-04)


Inducible copy number BioBrick plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4404
    Illegal NheI site found at 3317
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4404
    Illegal BglII site found at 1618
    Illegal BglII site found at 2665
    Illegal XhoI site found at 3462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4404
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4404
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4419
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1061
    Illegal NgoMIV site found at 3303
    Illegal AgeI site found at 937
    Illegal AgeI site found at 2596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

      • pSCANS via BNL ***

The pSB2 series plasmids are based on the pSCANS-5 plasmid from John Dunn's group at Brookhaven National Laboratory. The pSCANS plasmids are introduced <a href="http://www.genome.bnl.gov/Protocols/pscans.shtml">here</a>.


Source

MIT SBWG modification of pSCANS-5 from BNL

References