Part:pSB1K3:Design

High copy BioBrick assembly plasmid

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2183
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2189
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2183
  Illegal XhoI site found at 1033
  Illegal XhoI site found at 2059
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2183
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2183
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2198
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

Original Sequence

In Spring 2011, pSB1K3 was sequenced using several primers. The current sequence reflects the most recent sequencing of pSB1K3 (w/ BBa_J04450, located in SP 4000 Well 3A). However for posterity, we have included the old sequence that was originally specified in the Registry (PSB1K3-Archival-Seq-Text.txt) as well as images of the original sequence with markers pointing out the changes made:

Design Notes

Eliminated ampicillin resistance.

Source

This plasmid was derived from pSB1AK3-P1010.