Part:BBa_Z0251:Experience
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Applications of BBa_Z0251
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iGEM2020 Team Edinburgh |
iGEM 2020 TEam Edinburgh has tested successfully tested this promoter. It was ordered as a synthesized sequence rather than cloning it. It's efficiency was not assessed in comparison to other T7 promoters. Nevertheless it was used in the BBa_K3380500 construct (full description of the methods can be found under theexperience page of the BBA_K3380500 page) to express the iSpinach RNA aptamer (BBa_K3380500) flanked by F30 tRNA scaffolds (BBa_K3380101 and BBa_K3380102) some of the results can be seen below.
Figure 2. Impact of DFHBI fluorophore addition to the construct BBa_K3380500 over time. Each time point has 3 PCR tubes: the first one containing the construct + DFHBI fluorophore, second once having the fluorophore and no construct and the 3rd one having no T7 RNA polymerase added. The fluorescence was imaged under a Safe Imager™ Blue-Light Transilluminator (Invitrogen) with an amber filter unit and a phone camera. The details regarding the assembling process can be found in the Experience page of this construct[1]. |
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