Composite

Part:BBa_M36631:Design

Designed by: Benjamin Yeh, Jodie Sheffels, Ali Hoffer   Group: Stanford BIOE44 - S11   (2015-10-24)


AFP Preproprotein with FLAG Epitope


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct was made to be within a 1500bp limit with less than 5 repeats of >= 10bp, and no BsaI, BbsI, or BsmBI restriction sites.


Source

AFP protein derived from Aspergillus giganteus. See Part:BBa_M36623 for more details.

Standard FLAG epitope. See Part:BBa_M36629 for more details.

References