Generator

Part:BBa_K902041:Design

Designed by: Jeff Addison   Group: iGEM12_Calgary   (2012-10-24)

Constitutive AmdA Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 670
    Illegal XhoI site found at 162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
    Illegal NgoMIV site found at 1375
    Illegal NgoMIV site found at 1481
    Illegal AgeI site found at 548
    Illegal AgeI site found at 732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 406
    Illegal BsaI site found at 947


Design Notes

Please refer to the part design for the AmdA coding sequence.


Source

Part J13002 from the registry was constructed in front of an AmdA coding sequence that was amplified from Rhodococcus Erythropolis genomic prep via PCR.

References

Pavlova,J. and Maksimov,A. The ability of Rhodococcus erythropolis to transform amides. Laboratory of Chemical Mutagenesis,Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, 13 Golev Street, Perm 614000, Russia. 2011