Coding

Part:BBa_K902035:Design

Designed by: Jeff Addison   Group: iGEM12_Calgary   (2012-10-02)

AmdA Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 590
    Illegal XhoI site found at 82
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 90
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 1401
    Illegal AgeI site found at 468
    Illegal AgeI site found at 652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 326
    Illegal BsaI site found at 867


Design Notes

Nucleotide 72 was mutated from a G in the native organism to an A in our construct. This causes a silent mutation which does not affect the amino acid sequence.


Source

Gene was amplified using PCR from a Rhodococcus erythropolis strain 417 genomic prep template.

References

Pavlova,J. and Maksimov,A. The ability of Rhodococcus erythropolis to transform amides. Laboratory of Chemical Mutagenesis,Institute of Ecology and Genetics of Microorganisms, Ural Branch of RAS, 13 Golev Street, Perm 614000, Russia. 2011