Generator

Part:BBa_K880005

Designed by: Josh Atkinson, Mike Ferguson, and Ben Parker   Group: iGEM12_Michigan   (2012-09-29)

Strong promoter, strong RBS combination for high expression levels of proteins

The part is composed of sub-parts J23100 and B0034.

This is a strong promoter, strong RBS combination for high expression levels of proteins. It has a consensus constitutive promoter and RBS sequence-produces strongest possible expression.

Use of this Part by Team Michigan '12

Team Michigan '12 used this part as their primary protein-generator. Our data suggests that we were successful in using it to express FimE and HBiF recombinases for our recombinase-based switch systems. See our results page for more information[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters



Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 27.5 ± 8.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

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