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Part:BBa_K864404

Designed by: Pikkei Yuen   Group: iGEM12_Uppsala   (2012-09-25)

cjBlue Coding Device

This is an expandation of chromoprotein cjBlue BBa_K592011. It provides a turqoise color visible to the naked eye. The sequence is codon optimized for expression in E coli.

It has previously been assembled with another promotor (BBa_K592022).

Usage and Biology

This part is useful as a reporter.

CjBlue plate small.jpg UUChromo.jpg

iGEM12_Uppsala_University:
Left picture: Visual color expression of cjBlue with a different promotor(BBa_K592022).
Right picture: Pellet of chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Newcastle 2019 Characterisation

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Parameters
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