Reporter
redq

Part:BBa_K864502

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala_University   (2012-09-24)

RFP Coding Device, constitutive

Similar to the common RFP cassette BBa_J04450, but driven by a constitutive promoter. Useful for LacI positive systems or in LacIq backbones as a default insert, especially together with a high copy origin as in BBa_K864121.

When expressed in a high copy vector, the colonies are clearly red in color under natural light after about 18 hours. Smaller colonies are visibly red under UV. The RFP part does not contain a degradation tag and the RBS is strong.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None