DNA

Part:BBa_K863202

Designed by: Julia Schirmacher   Group: iGEM12_Bielefeld-Germany   (2012-09-23)

his4 gene from Pichia pastoris

The gene his4 from Pichia pastoris encode a trifunctional enzyme catalyzing the second (phosphoribosyl-ATP pyrophosphohydrolase), third (phosphoribosyl-AMP cyclohydrolase) and tenth (histidinol dehydrogenase) steps in histidine biosynthesis.

This part is a part of a shuttle vector (K863204) that could use for site directed recombination in the yeast P. pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 358
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 510


[edit]
Categories
Parameters
None