Part:BBa_K863122

BBa_I13522 with His-tag

GFP with constant promoter and His-tag made from BBa_I13522 (without the terminators). Originally designed to have a comparison to a His-tagged GFP to cellulose binding domain fusion protein.

Usage and Biology

GFP (mut3b) [note that this part does not have a barcode]

Tagged version of gfp from Repressilator reporter. See the design page for more source information.

Fluorescence wavelengths

Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -

• Excitation max - 501nm
• Emission max - 511nm

Latency

Cormack et al.Cormack report detectable fluorescence within 8 mins.

Pictures

• 

  BBa_I13522 visualized under non-UV lightbox

• 

  BBa_I13522 visualized under 254nm wavelength UV lightbox

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 813
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 724