Measurement

Part:BBa_K731711

Designed by: Giacomo Giacomelli, Anna Depetris   Group: iGEM12_UNITN-Trento   (2012-08-24)

BBa_K731710 backbone with T7terminator (BBa_K731721)

This is the BBa_K731710 platform with the BBa_K731721 T7 terminator inserted in the BioBrick cloning region. This part was used for the characterization of both BBa_K731721 and BBa_K731710. K731711image.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6865
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6865
    Illegal NheI site found at 6894
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6871
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6865
    Illegal BglII site found at 6011
    Illegal BamHI site found at 6847
    Illegal XhoI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6865
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6865
    Illegal XbaI site found at 6880
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 714
    Illegal NgoMIV site found at 1051
    Illegal NgoMIV site found at 4231
    Illegal NgoMIV site found at 4391
    Illegal NgoMIV site found at 5979
    Illegal AgeI site found at 6815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2228
    Illegal SapI.rc site found at 3310


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Parameters
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