Composite

Part:BBa_K575009

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-08-11)

LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP

This part consists of the LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter. It is a characterized as a unit within part BBa_K575028.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 827


Functional Parameters

n/aLasR/PAI1 Inducible Promoter + RBS (B0034) + GFP


Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 8.0 ± 4.3%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

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Categories
Parameters
n/aLasR/PAI1 Inducible Promoter + RBS (B0034) + GFP