Part:BBa_K554010

SoxR device

This part is composed by Constitutive Promoter linked to RBS+SoxR gene and Terminator.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

The Constitutive promoter+RBS+SoxR gene is used by [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil UNICAMP-EMSE Brazil team] in the [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer NO sensor device/ IL-10 producer] ("Device 2", which senses NO levels and responds by producing and secreting IL-10. This part is shown in the following schema:

Usage in testing Device 2 function

In order to test the ability of Jedi Bacteria in sensing NO levels and activating genes linked to SoxS promoter via SoxR transcription factor, we built a Device testing (Click here to see the experimental results),with SoxR constitutively transcribed and with GFP linked to SoxS promoter, as it is shown in the following schema (Figure 1):

Figure 1: Testing [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2] through replacement of IL-10 to GFP.

Source

Synthesized sequence and Registry entries.

References

1. Hidalgo E, Leautaud V, Demple B (1998) The redox-regulated SoxR protein acts from a single DNA site as a repressor and an allosteric activator. The EMBO Journal 17(9)2629–2636 [http://www.ncbi.nlm.nih.gov/pubmed/?term=9564045%20 Link to Pubmed]