Part:BBa_K5443002

Coumaric acid 3-hydroxylase (C3H)

Coumaric acid 3-hydroxylase (C3H) from Saccharothrix espanaensis, Sam5, functions as the second step in our vanillin biosynthesis pathway. It converts p-coumaric acid into caffeic acid via hydroxylation (Fig. 1).

This part builds on the work of earlier iGEM teams, who contributed C3H Parts as follows: BBa_I742105, BBa_K238007, and BBa_K2906013.

Figure 1. C3H-Sam5-catalysed conversion of p-coumaric acid to caffeic acid.
This reaction represents the second step in our vanillin synthesis pathway. The C3H enzyme Sam5 converts coumarate (p-coumaric acid) into caffeic acid.