Composite
rbs + sam5

Part:BBa_K238007

Designed by: Annelien Verfaillie   Group: iGEM09_KULeuven   (2009-07-27)


Sam5 gene with RBS

This is an improved version of the Sam5 gene (BBa_I742105). The restriction site for PstI that was initially found at location 1436 was mutated out. The mutagenesis was done by Mr. Rashed Al Naber under supervision of Prof. C. French of the University of Edinburgh, in March 2008, using BioBrick BBa_I742105 as a template (this BioBrick was prepared by the Edinburgh iGEM 2007 team but was not submitted to the Registry).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 45
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 968
    Illegal NgoMIV site found at 1370
    Illegal NgoMIV site found at 1374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 569


[edit]
Categories
Parameters
None