Intermediate

Part:BBa_K539621:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

a RBS (B0030)+ilvC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 1018


Design Notes

Built in weak expressing RBS (Part:BBa_B0030) and ilvC (Part:BBa_K539711). The sequence of ilvC had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.

Source

The ilvC is from the Escherichia coli str. K-12 substr. MG1655

References