Composite
CspB | RFP

Part:BBa_K525234:Design

Designed by: Timo Wolf   Group: iGEM11_Bielefeld-Germany   (2011-09-20)

Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 799
    Illegal PstI site found at 1125
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 799
    Illegal PstI site found at 1125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1876
    Illegal XhoI site found at 1332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 799
    Illegal PstI site found at 1125
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 799
    Illegal PstI site found at 1125
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 2088
    Illegal AgeI site found at 87
    Illegal AgeI site found at 680
    Illegal AgeI site found at 792
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1231
    Illegal AgeI site found at 1278
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1680
    Illegal BsaI.rc site found at 987
    Illegal BsaI.rc site found at 1365
    Illegal BsaI.rc site found at 1767


Design Notes

Gibson Assembly of BBa_E1010 and BBa_K525224 with linker Ala-Gly-Ala


Source

  • Gibson Assembly of BBa_E1010 and BBa_K525224 with linker Ala-Gly-Ala included in the primers
    • Primers for amplification of BBa_E1010
      • Fwd: TCCCGGCCATGGCGCAGGACGCTTCCTCCGAAGACGTTATCAAAG
      • Rev: TGGCAACCTGGGCGTCCTGTGCACCTGCAGCACCGGTGGAGTGACGACC
    • Primers for amplification of BBa_K525224 with backbone
      • Fwd: GGTCGTCACTCCACCGGTGCTGCAGGTGCACAGGACGCCCAGGTTGCCA
      • Rev: CTTTGATAACGTCTTCGGAGGAAGCGTCCTGCGCCATGGCCGGGA


References