Part:BBa_K5252006

XplB

XplB is an adrenodoxin reductase homologue [1] that along with XplB (BBa_K5252005) catalyses the degradation of RDX (Research Department eXplosive or 1,3,5-Trinitroperhydro-1,3,5-triazine) in Rhodococcus rhodochrous. The degradation mechanism is believed to involve denitration, followed by ring cleavage and mineralization, producing byproducts such as nitrite, formaldehyde, and formate. [2]

Adapted from BBa_K3857002 :

- Codon optimized for E. coli while removing SapI, BsaI, BbsI, PaqCI, Esp3I, FaqI restriction sites using Genscript.

- Addition of 3xFLAG-tag and GS-linker sequence

References

[1] Rylott EL, Jackson RG, Sabbadin F, Seth-Smith HM, Edwards J, Chong CS, Strand SE, Grogan G, Bruce NC. The explosive-degrading cytochrome P450 XplA: biochemistry, structural features and prospects for bioremediation. Biochim Biophys Acta. 2011 Jan;1814(1):230-6. doi: 10.1016/j.bbapap.2010.07.004. Epub 2010 Jul 17. PMID: 20624490.

https://pubmed.ncbi.nlm.nih.gov/20624490/#:~:text=XplA%20is%20a%20cytochrome%20P450%20that%20mediates%20the,that%20is%20fused%20to%20its%20flavodoxin%20redox%20partner.

[2] Seth-Smith HM, Rosser SJ, Basran A, Travis ER, Dabbs ER, Nicklin S, Bruce NC. Cloning, sequencing, and characterization of the hexahydro-1,3,5-Trinitro-1,3,5-triazine degradation gene cluster from Rhodococcus rhodochrous. Appl Environ Microbiol. 2002 Oct;68(10):4764-71. doi: 10.1128/AEM.68.10.4764-4771.2002. PMID: 12324318; PMCID: PMC126434.

https://pubmed.ncbi.nlm.nih.gov/12324318/

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1047
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 849
  Illegal NgoMIV site found at 1353
  
• 1000
  COMPATIBLE WITH RFC[1000]