Composite

Part:BBa_K5205004

Designed by: Yuqi Fu   Group: iGEM24_Hangzhou-SDG   (2024-09-24)


Non-OmpA-OsMTI-1b

This part consists of a cell membrane display system, Non-OmpA BBa_K5205003, fused with a rice metallothionein, OsMTI-1b (also known as OsMT1, BBa_K5205000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

The fusion of Non-OmpA with OsMTI-1b enables the metallothionein to be displayed on the E. coli cell surface, allowing the fusion OsMT1 to effectively bind heavy metal ions in the environment. We expressed this fusion protein in E. coli BL21 to turn it into a heavy metal remover.

Figure 1. SDS-PAGE of total protein of engineered E. coli BL21 expressing Non-OmpA-OsMTI-1b.

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