Composite

Part:BBa_K5198107:Design

Designed by: Robert Kaptur   Group: iGEM24_Duke   (2024-08-23)


nSEAP-NanoLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 571
    Illegal NgoMIV site found at 1540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 901
    Illegal BsaI.rc site found at 1390


Design Notes

The sequence was designed for extracellular secretion in mammalian cells.


Source

Refer to sources of parts BBa_K5198010BBa_K1734004


References