Intermediate

Part:BBa_K5181007

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-18)


Self sufficient araBAD promoter with RBS

General information: This part is a variant of the self-sufficient arabinose promoter part BBa_K5181006. The annotation of this part is as follows:

Annotation Range in basepairs length (bp) Misc. info
misc_DNA
1-9
9bp
 contains an NsiI cut site which includes an ATG 3 bp away from RBS
RBS
10-16
7bp
 Ribosome Binding site AAGGAGA(complement)
misc_DNA
17-51
35bp
 Gap between araBAD promoter and RBS
araBAD promoter
52-336
285bp
 arabinose induced promoters (complement)
misc_DNA
337-362
26bp
 Gap between araBAD promoter and araC gene
araC_gene
363-1241
879bp
 araC: Represses araBAD promoter in absence of L-arabinose
misc_DNA
1242-1271
30bp
 has 100% sequence similarity with pSEVA's MCS region and contains HindIII.

Part Image


This part can be used with pSEVA vectors to add an arabinose promoter with a strong RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 17
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 991


[edit]
Categories
Parameters
None