Intermediate

Part:BBa_K5181006

Designed by: Mayukh Mitra   Group: iGEM24_IISER-TVM   (2024-08-16)


Self sufficient araBAD promoter with RBS

General information: This part is a self sufficient arabinose promoter along with its Supporting gene AraC and RBS(Ribosome Binding site). The annotation of this part is as follows:

Annotation Range in basepairs length (bp) Misc. info
misc_DNA
1-30
30bp
 has 100% sequence similarity with pSEVA241 MCS region and contain HindIII,NotI and EagI sites
araC_gene
31-909
6bp
 araC: Represses araBAD promoter in absence of L-arabinose (complement)
misc_DNA
910-935
26bp
 Gap between araBAD promoter and araC gene
araBAD promoter
936-1220
285bp
 arabinose induced promoter
misc_DNA
1221-1256
36bp
 Gap between araBAD promoter and RBS
RBS
1257-1262
6bp
 Ribosome Binding site AAGGAGA
misc_DNA
1263-1272
10bp
 contains an NcoI cut site which includes an ATG 6 bp away from RBS

Part Image


This part can be used with pSEVA vectors to add an arabinose promoter with a strong RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 17
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 991


[edit]
Categories
Parameters
None