Generator

Part:BBa_K516210

Designed by: Nicolo' Politi, Susanna Zucca, Daniele Sartori   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

LuxI protein generator with Ptet and RBS B0030 (TERM-)


lux I protein generator driven by pTet promoter followed by BBa_B0030 high efficiency RBS.
The device is provided without Transcriptional Terminator. It could be possible use this part into plasmid vectors (such as pSB4C5) containing a Terminator in their backbones.
This is one of four systems with different RBSs provided to modulate the synthesis of the 3OC6-HSL autoinducer molecule ( the other parts are: BBa_K516211 with BBa_B0031 RBS ; BBa_K516212 with BBa_B0032 RBS ; BBa_K516214 with BBa_B0034 RBS).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aLuxI protein generator with Ptet and RBS B0030 (TERM-)