Coding

Part:BBa_K4721009

Designed by: Celeste Bos   Group: iGEM23_Leiden   (2023-10-11)


VanR repressor gene

This basic part was used to form a composite part, to create an vanillate inducible promoter system for Metylobacterium extorquens.

For the composite part and our aquired results, please visit BBa_K4721008.

Table 1: Overview of the basic parts forming the composite inducible PV10 vanillate inducible system.

Name Composite Part Composite Part Name Basic Parts Basic Parts Basic Part Features
PV10 vanillate inducible system BBa_K4721008 VanR repressor gene BBa_K4721009 Coding
RBS to combine with Pbla-mut1T promoter BBa_K4721013 RBS
Pbla-mut1T BBa_K4721012 Promoter
PV10 BBa_K4721011 RBS, Operator
RBS to combine with PV10 BBa_K4721010 RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 41
    Illegal NgoMIV site found at 198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 422
    Illegal BsaI.rc site found at 138
    Illegal SapI.rc site found at 688
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