DNA

Part:BBa_K4693004

Designed by: Vibhavari Medepalli   Group: iGEM23_IISER-TVM   (2023-10-10)


cDNA Cortisol 1

This is a short complementary DNA sequence that binds to 85-mer Cortisol aptamer (BBa_K4693004). Together with the aptamer, it forms an aptasensor that can quantify cortisol. Our team also designed this cDNA based on the inputs received from Dr. Tarun Kumar Sharma from Gujarat Biotechnology University, who suggested we design the cDNA to complement at least half the length of the aptamer, so this version of our cDNA 1 was 40bp long with delta G value -58.9. The 85-mer aptamer had a Kd value of - 16.1 nM and a delta G value of -10.62 kcal/mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None