Composite
Part:BBa_K4601254
Designed by: Doriane Blaise Group: iGEM23_Evry-Paris-Saclay (2023-09-23)
CmR expression cassette under the control of the J23110 promoter
This part is an expression cassette of the CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (BBa_K4601051) under the control of the BBa_J23110 constitutive promoter.
Usage and Biology
This part expresses constitutively the CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (BBa_K4601051). It can be used as a positive control in various experiments.
E. coli cells carrying it are resistant to the chloramphenicol antibiotic.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |