Coding

Part:BBa_K4601051

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


CmR

This part is CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (Uniprot P62577).

Usage and Biology

The chloramphenicol O-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic.

Figure 1. The mode of action of the chloramphenicol O-acetyltransferase enzyme: acetylation of chloramphenicol.

The CmR gene was isolated from the bacteriophage P1Cm carrying the Tncam-204 transposon derived from the R plasmid NR1 [1].


Reference

[1] Marcoli R, Iida S, Bickle TA. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS letters (1980) 110: 11–14.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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