Coding
Part:BBa_K4601051
Designed by: Doriane Blaise Group: iGEM23_Evry-Paris-Saclay (2023-09-23)
CmR
This part is CmR gene coding for the chloramphenicol O-acetyltransferase enzyme (Uniprot P62577).
Usage and Biology
The chloramphenicol O-acetyltransferase enzyme (EC 2.3.1.28) catalyses the conversion of chloramphenicol into chloramphenicol 3-acetate (Figure 1), thus inactivating the antibiotic.
Figure 1. The mode of action of the chloramphenicol O-acetyltransferase enzyme: acetylation of chloramphenicol.
The CmR gene was isolated from the bacteriophage P1Cm carrying the Tncam-204 transposon derived from the R plasmid NR1 [1].
Reference
[1] Marcoli R, Iida S, Bickle TA. The DNA sequence of an IS/-flanked transposon coding for resistance to chloramphenicol and fusidic acid. FEBS letters (1980) 110: 11–14.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |