Composite

Part:BBa_K4601244

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


AmpR expression cassette under the control of the J23110 promoter

This part is an expression cassette of the AmpR, an E. coli gene coding for a β-lactamase enzyme (BBa_K4601041) under the control of the BBa_J23110 constitutive promoter.

Usage and Biology

This part expresses constitutively the AmpR, an E. coli gene coding for a β-lactamase enzyme (BBa_K4601041). It can be used as a positive control in various experiments.

E. coli cells carrying it are resistant to β-lactam antibiotics like ampicillin or carbenicillin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None