Coding

Part:BBa_K4601041

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


AmpR

This part is AmpR, an E. coli gene coding for a β-lactamase enzyme. It is a derivative of the TEM-1 β-lactamase (Uniprot P62593) with two mutations V82I and V182A. In addition, compared to the sequence found in pSB1A3 from which it was derived by PCR amplification, two synonymous mutations Y44 (TAT->TAC) and S240 (TCC->TCG) were detected.

Usage and Biology

AmpR is a protein associated with the resistance mechanism against the large family of β-lactams, antibiotics which include the well known penicillin and ampicillin.

β-Lactamases act by hydrolysing the β-lactam ring (Figure 1), thus inactivating the antibiotic.

T--Evry Paris-Saclay--beta-lactam ring hydrolysis.png

Figure 1. The mode of action of β-lactamases: hydrolysis of the β-lactam ring.

Fortunately, among all the subgroups of β-lactams (table 1), some have less affinity with the β-lactamase(s) and overcome this resistance.

Table 1. β-lactam groups and sub-groups, classified based on their core ring structures.
penams carbapenams clavams penems carbapenems cephems carbacephems oxacephems monobactams
T--Evry Paris-Saclay--penam.png
T--Evry Paris-Saclay--carbapenam.png
T--Evry Paris-Saclay--clavam.png
T--Evry Paris-Saclay--penem.png
T--Evry Paris-Saclay--carbapenem.png
T--Evry Paris-Saclay--cephem.png
T--Evry Paris-Saclay--carbacephem.png
T--Evry Paris-Saclay--oxacephem.png
T--Evry Paris-Saclay--monobactam.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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