Translational_Unit

Part:BBa_K4158011

Designed by: Nanami Onishi, Eri Ito   Group: iGEM22_Waseda_Tokyo   (2022-09-22)


RBS-GFP

This part contains RBS and GFP coding site. Especially, used for construction of the plasmid PedcR-RBS-GFP, our project device with PedcR (BBa_K4158013).

Information of the sequence was from a previous paper[1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 658


References

1. Ibero J et al. Identification of the EdcR Estradiol-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor. Genes (Basel). Genes (Basel). 12(12):1846(2022).

[edit]
Categories
Parameters
None