Regulatory

Part:BBa_K4158013

Designed by: Nanami Onishi, Eri Ito   Group: iGEM22_Waseda_Tokyo   (2022-09-25)


PedcR

This part contains a promoter regulated by EdcR and 17β-Estradiol((1S,3aS,3bR,9bS,11aS)-11a-Methyl-2,3,3a,3b,4,5,9b,10,11,11a-decahydro-1H-cyclopenta[a]phenanthrene-1,7-diol).

Information of the sequence was from a previous paper[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 145
    Illegal SapI site found at 9
    Illegal SapI site found at 37


1. Ibero J et al. Identification of the EdcR Estradiol-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor. Genes (Basel). Genes (Basel). 12(12):1846(2022).

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Categories
Parameters
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