Composite

Part:BBa_K4012029

Designed by: Jiayi He   Group: iGEM21_AISSU_Union   (2021-10-20)


RtTAL-Pc4CL-GbCHS-MsCHI level2

It is a composite part of naringenin metabolic pathway. pRET2-RtTAL-tSSA1-pPGK1-Pc4CL-tCYC1-pTEF1-GbCHS-tADH1-pPOP6-MsCHI-tTDH1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 714
    Illegal BglII site found at 2758
    Illegal BglII site found at 4201
    Illegal BglII site found at 6848
    Illegal BglII site found at 9038
    Illegal XhoI site found at 2844
    Illegal XhoI site found at 8072
    Illegal XhoI site found at 9746
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 50
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI site found at 2830
    Illegal BsaI site found at 3099
    Illegal BsaI site found at 6137
    Illegal BsaI site found at 8058
    Illegal BsaI site found at 8327
    Illegal BsaI.rc site found at 724
    Illegal BsaI.rc site found at 3080
    Illegal BsaI.rc site found at 4211
    Illegal BsaI.rc site found at 6858
    Illegal BsaI.rc site found at 8308
    Illegal BsaI.rc site found at 9048
    Illegal SapI site found at 7762
    Illegal SapI.rc site found at 7711


Level2 plasmid assembly

Figure 1: [1] A: The results of colony PCR of upstream transformant; B: The results of colony PCR of downstream transformant; C: Construction of catechin metabolic pathway; D：Construction of naringenin metabolic pathway

Assembly of synthesis pathway of naringenin, shown by Fig.1.

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