Part:BBa_K3977006

3WJ 4XBRO

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 199
Illegal SpeI site found at 174
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 199
Illegal SpeI site found at 174
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 199
Illegal BamHI site found at 168
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 199
Illegal SpeI site found at 174
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 199
Illegal SpeI site found at 174
1000
COMPATIBLE WITH RFC[1000]

Contribution

Group: SCU-China iGEM team 2021
Author: Yilong Xu
Summary: Improve this part by expanding itself
'Link to our biobrick: https://parts.igem.org/Part:BBa_K3977006

Characterization/Improvement

Based on BBa_K3380153, we construct a RNA adaptor with 4 repeats of BBa_K3380153, We put them under J23100, and detect their performance in 488/527.

Our result shows that BBa_K397706 show more output (C) , meaning that it performed better than original part BBa_K3380153 and our improvement is successful.

                        Figure 1: Relative fluorescence intensity BBa_K3380153 and BBa_K3977006