Part:BBa_K389423:Design

VirA/G signaling system + sensitivity tuner + luciferase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 647
  Illegal NheI site found at 2581
  Illegal NheI site found at 2604
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1632
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 5029
  Illegal NgoMIV site found at 6373
  Illegal NgoMIV site found at 6394
  Illegal AgeI site found at 4552
  Illegal AgeI site found at 4664
  Illegal AgeI site found at 4866
  Illegal AgeI site found at 6097
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1768
  Illegal SapI.rc site found at 6279
  

Design Notes

a modified sensitivity tuner BBa_I746390 to enhance the luciferase signal which interacts as a reporter for a VirA/G signaling system

Source

• luciferase from pGL4.10[luc2] (Promega) (BBa_K389004)
• terminator, RBS and constitutive promoter from the parts.igem (BBa_B0017, BBa_B0034, BBa_J23110)
• sensitivity tuner part consists inter alia of
  • delta activator from phage phiR73 (BBa_I746352)
  • PF promoter from phage P2 (BBa_I746360)
• virG was synthesized by Mr. Gene (BBa_K389002)
• virA and vir promoter from Agrobacterium tumefaciens C58, TI-plasmid (BBa_K389001, BBa_K389003)

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]