Composite
Part:BBa_K389013:Design
Designed by: Jonas Aretz Group: iGEM10_Bielefeld-Germany (2010-09-11)
VirA reporter system mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1686
Illegal AgeI site found at 1798 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
- medium strong constitutive promoter
- The virG gene is mutated so it works without an rpoA subunit from Agrobacterium tumefaciens (YC Jung et al., 2004)
- double terminator (forward) to keep expression of mRFP by the constitutive promoter low
- mRFP gene to show the activity of the vir promoter
Source
- virG gene synthesized by Mr. Gene (BBa_K389002)
- vir promoter from A. tumefaciens C58 (BBa_K389003)
- constitutive promoter, double terminator and mRFP from parts.igem (BBa_J23110, BBa_B0017, BBa_E1010)
References
YC Jung et al. (2004) Mutants of Agrobacterium tumefaciens virG Gene That Activate Transcription of vir Promoter in Escherichia coli, Current Microbiol 49:334-340.